Associate professor of cultural anthropology at Kansas State University, Michael Wesch, has defined YouTube poops as 'absurdist remixes that ape and mock the lowest technical and aesthetic standards of remix culture to comment on remix culture itself'. In many cases, YouTube poops utilize a bizarre sequence of elements which may entertain, confuse, or irritate, depending on the viewer. To this degree, a YouTube poop may even consist solely of an existing video, sometimes modified, repeated in a slowed or remixed loop. Some of these videos may involve completely or partially repurposing sources to create or convey an often self-aware story, while others follow a non-linear narrative, and some may contain no storyline at all, instead regarded among the lines of surreal humour and artistic experimentation. The most common of such rearrangement being 'sentence-mixing', a form of editing in which dialogue is rearranged or chopped up to form new, often humorous dialogue-drawing humour from the unexpected changes to usually well-known source dialogue.

A typical YouTube poop uses visual and auditory effects to alter the underlying work, as well as rearrangement of individual clips. Media sources of YouTube poops include television shows, movies, anime, cartoons, commercials, video games, music videos, live performances, historical footages and other videos obtained from YouTube or elsewhere. Meen became incredibly common sources in YTPs, as they had been ironically acclaimed for deviating from the quality expected of their respective franchises. Between 20, cutscenes from games released on the Philips CD-i platform (most notably Hotel Mario, Link: The Faces of Evil, and Zelda: The Wand of Gamelon) as well as the animated series Adventures of Sonic the Hedgehog, Super Mario World and the 1995 DOS game I.M. It exhibits stylistic and aesthetical staples of YouTube poop, including repetition of clips for comedic effect (the video name is a particularly campy pun spoken by Luigi that is repeated throughout the video), and critically disregarded media as a video source. 3, which has been regarded as kitsch by fans of the Mario franchise, as a primary source. It was created with Windows Movie Maker and uses clips from the 1990 animated television series The Adventures of Super Mario Bros. 3 REMIXED!!!', or what is now known as 'I'D SAY HE'S HOT ON OUR TAIL', by YouTube user SuperYoshi. The video regarded as the first YouTube poop was uploaded on the website SheezyArt, as opposed to YouTube, circa 2004: 'The Adventures of Super Mario Bros.

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